Abstract

Abstract : Skin was mounted by sandwiching between clamped donor and receptor compartment. The receptor compartment was filled with freshly prepared buffer. All air bubbles were expelled from the receptor by inverting the diffusion cell then allow the bubbles to travel out of the sample port. A aliquot (1ml) of donor solution was placed on the skin, while the receptor fluid was kept under stirring. After predetermined time interval 3ml of sample was drawn from receptor cell and replaced with fresh buffer. The sample was analyzed for drug content permeated by measuring absorbance at 276 nm in a UV Spectrophotometer. Low bioavailability of drug through skin is one of the major obstacles to the development of Trandermal drug delivery products. Several natural polymers have been studied for permeation effect. Majority of them were found to produce undesirable effect on skin or body. So for the overcome this problem we can use different ratio of natural polymers to optimize the best permeation of repaglinide through the rat skin.