Abstract

Abstract : The genuineness of the plant species was identified by referring the deposited specimen by Botanical Survey of India, Gangtok, Sikkim. The plant material was air dried and macerated using methanol as solvent. The extract dried and used for further study. Different concentration (100, 300, 500 and 1000 μg/ml) of standard (Acarbose) and methanol extract of Aeschynanthus sikkimensis (Clarke) Stapf. were used for the study. The absorbance values were taken in spectrophotometer at 565nm and 405nm for α-amylase and α-glucosidase enzyme, respectively.
Result
The methanol extracts of Aeschynanthus sikkimensis (Clarke) Stapf. inhibit the enzyme α -amylase and α-glucosidase enzyme in concentration dependent manner. The IC50 values of methanol extract of Aeschynanthus sikkimensis (Clarke) Stapf. was 420± 4.09 and standard drug Acarbose was 159± 8.01 for α –amylase. In case of α-glucosidase the IC50 value for methanol extract of Aeschynanthus sikkimensis (Clarke) Stapf. was 78± 3.24 and standard drug Acarbose was 35.4± 5.39.
Conclusion
The Current Study clearly indicate that inhibition of enzyme α -amylase and α-glucosidase enzyme in concentration dependent manner by methanol extract of Aeschynanthus sikkimensis (Clarke) Stapf. The findings indicate the methanol extract of Aeschynanthus sikkimensis (Clarke) Stapf. have in vitro anti diabetic activity.